コレラ菌検索に対する螢光抗体法の応用

抄録

1. Both El Tor and Asiatic cholera vibrios were stained specifically with anti-El Tor vibrio fluorescent antibody irrespective of their types, whereas twenty laoratory strains of Gram-negative rods. other than cholera vibrio could not be stained with the same fluorescent antibody.
2. In the fecal specimens from the non-cholera patients and healthy persons, the fluorescent antibody stain-positive organisms or particles were found in a very high rate (88%), in which only a few were strongly positive. These positively stained organisms were easily differetiated from cholera vibrio in the morphological properties or strength of fluorescence. The differentiation of specific and non-specific stain was improved by the observation of the same field using the dark-field illumination by the visible light.
3. The limit of bacterial concentration in this technique to find the stained organisms obviously in the smeared specimen, was considered as around 10⁶/ml.
4. After the enrichment culture for 6 hours, the vibrios could be detected by fluorescent antibody staining in the material which initially contained vibrios as few as about 10²/ml.
5. Fluorescent antibody technique is considered as an effective screening method to detect cholera, patients or carriers, as this technique requires shorter time than the ordinary isolation technique.