Isolation of Streptomycin-resistant Salmonella Strain, Salmonella give

Abstract

On September 4th, 1964, drug-resistant salmonella strain was isolated from a patient (Hibino) suffering from slight fever and several diarrhoeae.
The bacilli were identified morphologically, biologically and serologically as salmonella give. The resistance of the strain for usual antibiotics was for streptomycin 250 mcg/ml, for chloramphenicol 3.13 mcg/ml, for tetracycline 25 mcg/ml, whereas for sulfonamide that was 100 mg/di sensitive.
By mixed culture method after Ochiai and Akiba only streptomycin resistance was transfered to sensitive E. coli K-12. And the transmissed resistance of E. coli K-12 was furthermore transfered to the sensitive standard strain of salmonella give.
It may be said that the isolation of drug-resistant salmonella from a human being without foregoing medication of any antibiotics is the first cace in Japan.