Detection of Neisseria gonorrhoeae Antigens by an Enzyme Immunoassay (Gonozyme)
1985 Volume 59 Issue 12 Pages 1236-1240
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1985 Volume 59 Issue 12 Pages 1236-1240
An enzyme immunoassay (EIA) (Gonozyme, Abbott Laboratolies) was evaluated by a correlation of optic density (OD) reading by EIA with number of organisms of a clinical strain. According to our experiments, 103 CFU/ml of N. gonorrhoeae was shown positive at OD reading of 0.489, and 105 CFU/ml was detected at OD reading of≥2.0.
Results of EIA were compared with culture techniques and direct Gram stain (GS).Samples studied were prepared from exudate of urethral tract of 261 males and cervix of 58 females who attended a VD clinics.Compared with culture techniques, EIA had 100% and 95.5% agreements of positive results in males and females, respectively.In spite of high agreements of positive results, agreements of negative results were low: 16.7% in males and 69.4% in females .This is probably due to long duration between collection of specimens and inoculation for culture, because the length of duration was associated with low agreements.Results of EIA and cultures on the day when specimens were collected were agreed completely.Positive agreements of EIA with GS were high, 98.8%, in males and relatively low, 76.9%, in females.A low agreement was considered to be due to error of GS.Agreements of negative results were 100% in males and 94.7% in females.
Our experiments suggested that EIA be an attractive alternative as the rapid, accurate diagnosis to GS of woman patients and culture when specimens were needed to transport.
