1985 Volume 59 Issue 12 Pages 1231-1235
Useflueness of Fluorescent-antibody (FA) test using monoclonal antibodies to herpes simplex virus (HSV) type 1 and 2 was tested for rapid detection and serotyping of HSV.
1) Serotyping of HSV: For evaluation of specificity of FA test, 52 isolates were used. FA test showed that 31 of 41 isolates from genital tracts were HSV-1 and 10 were HSV-2, and all of 11 isolates from vesicle fluid were HSV-1.These results completely coincided with the results obtained by other methods; neutralization test, biological marker test using RL-33 cells and electrophorogram of type specific DNA.
2) Rapid detection of HSV: To examine sensitivity of FA test, MRC-5 cells were infected with serial dilution of HSV, and then harvested at 24 hr intervals after infection.FA test could detected HSV antigen as early as 24 hr after infection when 103-104 PFU/ml of HSV were inoculated to MRC-5 cells. When the cells were infected with 101-103 PFU/ml of HSV, HSV antigen were detectable 48-96 hr after infection.
3) Clinical specimens from genital tracts were inoculated to MRC-5 cells which were then centrifuged for enhancement of virus isolation rate.In all of 10 specimens tested, HSV antigen was detected and serotyped by FA test at 24 hr after infection.
