1988 Volume 62 Issue 12 Pages 1152-1156
Killing effects of extracts from 12 kinds of foods and ethanol on Anisakis larvae were examined to find prophylaxis. Saline extracts from freeze-dried Perilla frutescense viridis Makino (5% w/v), Zingiber officinale (5%), Wasabia japonica (5%), Allium sativum (5%), Z. officinale (2.5%), W. japonica (2.5%), and ethanol (8% v/v) were effective to stop the motion of worms (without movement for 1 min even though stimulated by tweezers) with mean durations of 3.1 hr, 3.2 hr, 5.6 hr, 10.8 hr, 11.6 hr, 17.6 hr and 2.0 hr, respectively. Larvae contacted with these extracts for 24 hr lost mobility (observations for more than 4-5 hr with stimulations) completely. Further dilutions of these extracts or 5% extracts (w/v) from Allium fistulosum, Petroselinum sativum, Raphanus sativus, Brassica oleracea, Spinacia oleracea, Laminaria angustata, Capsicum annuum, and Thea sinensis, had little effects.
Killing effects of well-known components of P. frutescens and Z. officinale were examined, too. Minimum doses of [6]-shogaol, [6]-gingerol, perillaldehyde and perillyl alcohol for complete killing of larvae were 62.5, ug/ml, 250, ug/ml, 125 pg/ml and 250 dug/ml, respectively. Thiabendazole (10 mg/ml) had no killing effect.
