A case study of various histological changed minute hepatocellular carcinoma with special reference to ultrastructural observation

Abstract

A 64-year-old male with alcoholism was admitted to our hospital because of ascites. Diagnosis of liver cirrhosis was made based on clinical data and celiac angiography revealed tumor stain in the right hepatic lobe. Surgical enuclation of the tumor was carried out and light and electron microscopic study of the resected tumor (1.7×2.0×2.2cm diameter) was made.
Histological findings showed the encapsulated tumor producing the bile and septal formation in some area of the tumor. The tumor mainly consisted of clear cells, but some parts revealed pseudoglandular pattern. Both globular and reticular hyaline bodies revealed the same staining pattern following luxal fast blue stain. Azan-Mallory's stain and PAS stain in the tumor cells.
Electron microscopy showed that these bodies were composed of conglomerated fibrils with parallel array and randomly oriented arrangements. No membrane structure was detected arround the Mallory bodies and the fibrils revealed 100-200Å in diameter. No tumor cells derived from bile duct cells were detected in pseudoglandular pattern.
Morphogenesis of the bodies in relation to minute hepatocellular carcinoma remains to be clarified.