Effect of acute ethanol administration on the cytoskeleton of rat hepatocyte

Including the comparison of the two kinds of perfusion studies

Abstract

In order to examine the cytoskeleton in normal rat hepatocyte and the effect of acute ethanol administration on the cytoskeleton of rat hepatocyte rat livers were perfused with 0.5% Triton X-100 or 0.15% Triton X-100 for 120min. In ethanol group rat 5g/kg b.w. ethanol were given intragastrically. Microtubles and intermediate filaments were easily observed by this two kinds of perfusion studies; however, it was difficult to observe microfilament in this two kinds of perfusion studies. No significant morphological changes were noted in the microtubles and the intermediate filaments in the rat hepatocytes received acute ethanol administration compared with the control rats.