Hyperthermo-chemotherapy of in vivo isolated liver in the rat

Abstract

This study presents a model for the in vivo perfusion of the isolated rat liver with either cytotoxic agents or hyperthermic saline administered either through the hepatic artery or the portal vein.
Rats were able to survive following the perfusion with a dose of up to 0.25g/kg of 5-FU and 2.0-2.5mg/kg of Mit-C by both routes.
Regional hyperthermic hepatic perfusion produced temperature- and time-dependent hepatotoxic effects. A marked elevation in SGOT and SGPT levels was observed 24 hours after perfusion, but was reversible.
³H-TdR incorportion into hepatic DNA 24 hours after partial hepatectomy was severely inhibited by prior hepatic perfusion with either cytotoxic drugs or regional hyperthermia. This in vivo isolated hepatic perfusion technique could be applicable for assessing the effect of thermochemotherapy on tumor growth in the liver.