Biochemical analysis of liver regeneration following after galactosamine-induced liver cell injury

Abstract

Simultaneous administration of glucagon and insulin has shown to stimulate DNA synthesis in liver cell, both in vivo and in vitro studies. However, it is fairly known whether synergistic action of these pancreatic hormones play an important role in the process of liver regeneration or repair in the rats carrying severe liver injury. Therefore, in order to clarify the interaction of both hormones to injured rat liver, galactosamine in a dose of 1, 000mg/kg b.w. was administered intraperitoneally into the fasted animals, and these were killed at several critical points for studing on changes of pancreatic hormones in plasma, cyclic nucleotides of liver tissues. ODC activity and number of glucagonreceptor on plasma membrane isolated from these livers. These parameters were depressed in the process of cell necrosis with galactosamine, while those were restored in phase of repair or regeneration nearly to the levels of the control. From these results, it will be concluded that administration of pancreatic hormones might be reasonable for increase of the capacity to regenerate even in the chemically induced rat liver injury.