Changes in plasma nucleoside levels during hepatic regeneration in partial hepatectomized and CCl₄damaged rats

Abstract

The levels of plasma nucleosides were measured with HPLC in the rats during hepatic regeneration following partial hepatectomy and CCl₄-induced acute liver injury. After hepatectomy, cytidine (Cyd) and adenosine (Ado) were significantly increased at three and six hours, preceding the peak of transaminases. At 36, 48, and 72 hours after the operation, Cyd and urindine (Urd) were significantly decreased, while the increase in mitotic index was observed. At 24 hours after administration of CCl₄, Cyd, Urd, Ado, pseudouridine (Ψrd), and 1-methyladenosine (m¹A) were increased, preceding the elevation of transaminases. At 72 hours, Cyd was significantly decreased, while mitotic index was increased. These results suggest the relationship between reduction of plasma nucleoside levels and hepatic regeneration.