In vitro simple determination of resistance to bacterial wilt of carnation

Abstract

In vitro simple method for determination of resistance to a pathogen of bacterial wilt of carnation (Burkholderia caryophylli) was developed. The bottom of unrooted cuttings of carnation was dipped in bacterial suspension (2.7×10²-10³cfu/ml) for five min, rinsed with distilled water for ten min, and then incubated with distilled water in test tubes at 30°C. The symptons of bacterial wilt can be visible within two weeks after the inoculation. In the method using unrooted cuttings, the level of resistance of carnation to B. caryophylli was efficiently determined.