Abstract
The emulsified solution of Folidol E-605 was given subcutaneously on the back skin of 51 albino rats in amount ranging from 1.0 to 6.0mg pro kilogram body weight thereby to induce either the chronic, subacute or acute poisoning in proportion to the doses given. The changes of the tooth and the surrounding tissues from the first day to the sixth month of the administration were consecutively followed up patho-histologically and the results were summarized as follows. 1) The body weight either reduced or slightly increased in the subacute poisoned groop, while in the chronic poisoned groop given 3mg of Folidol the body weight first reduced, then increased steadily. 2) The pathologic changes similar to those induced in the internal organs of the acute poisoned animals were also observed occurring in the chronic poisoned animals. 3) Of the histological findings in the tooth and the surrounding tissues, the-following changes are to be noted. a) Between the formative layer of ameloblasts and the young enamel substance insoluble in the solution of acids one noticed drop-like, granular or cuboidal foreign materials deposited. No changes, however, were seen in the tooth enamel. b) Only in the subacute and chronic poisoned groops were found the pathologic changes in the ameloblast layer. In the layer from its formative end to the region dissolved by the acids, the inner structure of cells was either irregularly oriented or even disintegrated. The outer ameloblast layer undergoing atrophy lost its pectinated epithelial papillae. and the concaved portions lying between the papillae showed vacuolar degeneration. In some specimens hypertrophy of the epithelial papillae took place, resulting disapperance of the concaved portions. In the intermediate layer of the ameloblast at the gingival margin of alveolar process, vacuolar degeneration and cyst formation were observed. c) Blood congestion and bleedings of irregular dimensions occurred in the pulp tissue, especially in those of the molar teeth. In the severe cases of the poisoning the pulp assumed edematous appearance accompanying atrophy of the ground cells. Further there occurred decreased staining reaction of the odontoblast, atrophy of the cell nuclei, irregular arrangement of the odontblast layer and its vacuolar degeneration. In the pulp of molar teeth parts of the cells underwent nucleus disintegration and finally their disappearance. In the dentin a slight disturbance in calcification, showing itself like the stratus, was observed, which was more frequent in the dentin of molar teeth. d) The pathological changes in the tooth sac and the periodontal membrane occurred exclusively in the incisors, showing congestion as well as edematous loosenning of the connective tissue surrounding blood vessels in the membrane. e) Otherwise there were found in the enamal, gingiva and alveolar bone no remarkable changes whatever. 4) It was concluded from our results that Parathion seemed to exercise moderate effects on the embryo and suckling of the animal by way of the placenta and breast feeding. It was demonstrated that the similar moderate degeneration affecting the mother animals under the poisoning occurred to the offsprings in the kidney, liver as well as in certain regions of the salivary glands. These, however, were not found in the tooth and the surrounding tissues.
