Abstract
We previously reported that ultrasound-mediated destruction of microbubbles might be an innovative non-invasive drug delivery system. The anticancer drug doxorubicin is a potent anticancer drug that is used for many types of malignancies；however, the severe side effects associated with use of free doxorubicin has limited its clinical use. Although encapsulated doxorubicin liposomes (Doxil) strongly reduced the cardiotoxity of doxorubicin, other side effects were reported and release of doxorubicin from tumors after accumulation is difficult. We prepared encapsulated doxorubicin bubble liposomes (EDBL) from Doxil with the aim of improving cytotoxicity of Doxil for drug delivery with ultrasound. Human gingival cell carcinoma Ca9-22 cells were exposed to ultrasound in the presence of Doxil or EDBL. Cell viability was determined by trypan blue staining and evaluated by WST-8 assay. Apoptosis was evaluated using flow cytometry and Hoechst dye 33342 staining. The combination of EDBL and ultrasound enhanced the cytotoxicity and apoptosis of Ca9-22 cells. Sonoporation is a potent method to deliver doxorubicin into Ca9-22 human oral cancer cells. Our in vitro results indicate that EDBL could be a useful tool to achieve efficient ultrasound-controlled doxorubicin delivery in vivo.
https://ror.org/03bwzch55 
