EXPERIMENTAL STUDIES ON THE MECHANISM OF BACTERIOSTATIC ACTION OF ISONICOTINIC ACID HYDRAZID (INAH)

Report 2. INFLUENCE OF INAH ON THE DEHYDROGENASES OF BCG

Abstract

Using the Thunberg's methylene blue decoloration method, the influence of INAH and its derivatives on the dehydrogenation of various substrates by BCG suspension were examined. Following results were obtained:
1. The inhibitory influence of INAH was observed in the dehydrogenation of lactic acid, malic acid, glucose, formic acid, ethyl alcohol, glutamic acid and pyruvic acid by BCG suspension, on the other hand, in case of histidine and succinic acid, it was not found.
2. The inhibitory influence of INAH on the dehydrogenation of lactic acid by BCG suspension was decreased to some extent by the addition of a large amount of cozymase.
3. The inhibitory influence of INAH on the dehydrogenation of various substrates by BCG resistant to 100 mcg/ml INAH was found to be remarkably weak.
4. N-isonicotinyl-N'-glucosyl hydrazine and N-isonicotinyl-N'-isopropyl hydrazine inhibited the dehydrogenation of various substrates by BCG suspension, however, they were weak compared with INAH.