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Volume 29, Issue 6
Displaying 1-7 of 7 articles from this issue
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Report 2. INFLUENCE OF INAH ON THE DEHYDROGENASES OF BCGMINORU YAMAMOTO1954 Volume 29 Issue 6 Pages 195-199
Published: 1954
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSUsing the Thunberg's methylene blue decoloration method, the influence of INAH and its derivatives on the dehydrogenation of various substrates by BCG suspension were examined. Following results were obtained:
1. The inhibitory influence of INAH was observed in the dehydrogenation of lactic acid, malic acid, glucose, formic acid, ethyl alcohol, glutamic acid and pyruvic acid by BCG suspension, on the other hand, in case of histidine and succinic acid, it was not found.
2. The inhibitory influence of INAH on the dehydrogenation of lactic acid by BCG suspension was decreased to some extent by the addition of a large amount of cozymase.
3. The inhibitory influence of INAH on the dehydrogenation of various substrates by BCG resistant to 100 mcg/ml INAH was found to be remarkably weak.
4. N-isonicotinyl-N'-glucosyl hydrazine and N-isonicotinyl-N'-isopropyl hydrazine inhibited the dehydrogenation of various substrates by BCG suspension, however, they were weak compared with INAH.View full abstractDownload PDF (1060K) -
K. KURAKANE, H. TAKASAKI1954 Volume 29 Issue 6 Pages 200-204
Published: 1954
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSThe potency of 2 samples of the BCG purified tuberculin (BCGPT) obtained after Kurakane's (one of the authors) procedure, was investigated on 1399 persons who had not been vaccinated with BCG and 944 persons who had been vaccinated in comparison with the respective findings of the old tuberculin (0.1 ml of a 1: 2, 000 dilution) (OT) standardized by the National Institute of Health in Tokyo, and the following results were obtainedView full abstractDownload PDF (1183K) -
(EXPERIMENTAL STUDIES ON THE TUBERCULOUS ALLERGY: II REPORT)SHIGERU NAKAMURA, YASAKA OGAWA, MINORU HIRAYAMA, KEIICHIRO TAKA, SHIGE ...1954 Volume 29 Issue 6 Pages 205-210
Published: 1954
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSIn the 1st report, the method to produce the experimental tuberculous cavities in rabbit's lung which was successful in high percentage was reported. In this article, the pathohistologic findings of these cavities are reported.View full abstractDownload PDF (4508K) -
PART I. COMPARISON OF THE TUBERCULIN REACTIONS BETWEEN THE FLEXOR SURFACE AND THE EXTENSOR SURFACE OF THE FOREARM WITH OT IN TWO DIFFERENT DILUTIONS OF 1: 2, 000 AND 1: 10. 000TSUNEO ITOH, MICHIAKI MAEDA, KEN YANAGISAWA1954 Volume 29 Issue 6 Pages 211-215
Published: 1954
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSVariability of tub3roulin reactions depending on the difference in the locality of the skin tested, has been already reported by many workers. Tuberculin reactions tested on the flexor surface and the extensor surface of the forearm were compared, for in our country the test has been made on the flexor surface while the workers of WHO advocate the other side.
Children of primary and secondary schools totaling 2, 448 were divided into two groups. One group received four injections of each 0.1 ml on the flexor and extensor surfaces of both forearms, the left with the 1: 2, 000 dilution and the right with the 1: 10, 000 dilution of OT. The other group was treated likewise except that the left side forearm received the 1: 10, 000 dilution and the right side 1: 2, 000 dilution of OT. Readings were made 48 hours later, the results of which were as follows:
1. The grade of positivity as well as the intensity of redness was better on the flexor surface than on the extensor surface on both left and right forearms and their distribution was also different.
2. The intensity and frequency of induration, on the contrary, were stronger and higher on the extensor surface than on the flexor surface on both forearms. There were 34.7% induration positive cases by 1: 2, 000 dilution on the extensor surface while the flexor surface showed no reaction.
3. The formation of double redness was also higher on the extensor surface than on the flexor surface.
4. Except some difference in intensity, the reactions responded to the 1: 10, 000 dilution of OT were of the same tendency.View full abstractDownload PDF (914K) -
OSAMU SEKINE1954 Volume 29 Issue 6 Pages 216-220
Published: 1954
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSAdvantage in the use of supersonic wave as a homogenizer for the preparation of BCG vaccine lies in the relative easiness of producing a large quantity of concentrated emulsion with good dispersibility of the bacilli in a rather short time.View full abstractDownload PDF (1164K) -
TATSUICHIRO HASHIMOTO, KONOSUKE YOSHIDA1954 Volume 29 Issue 6 Pages 221-224
Published: 1954
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSSince the standard strains of human type of tubercle bacilli are not always constant in their viru lence, the experimental studies on their virulence often encounter with great dfficulties. In order to analyse the factors influencing on their virulence, guinea pigs were inoculated with H2 or H37Rv subcu taneously and autopsy findings were examined.
The results revealed that it was impossible to estimate the degree of their virulence from the standpoint of the size of inoculum (semidry weight or viable units) or culture days etc., because of the unexpected qualitative variations in their virulence in every generation cultivated on potato-media. The instability in their virulence may due to the irregular variation in the population of lowly virulent mutants influenced by some unknown conditions.
As a practical method adequate for the stabilization or their virulence, therefore, freeze-drying procedure was adopted which enabled to fix the population of highly virulent bacilli. Guinea pig inoculation showed that these freeze-dried strains maintained their virulence fairly well being unchang ed even 10 months after drying. This freeze-drying method made it possible to undertake animal experiments with virulent bacilli of known virulence and viable units.View full abstractDownload PDF (1031K) -
MASAO MOTOHASHI1954 Volume 29 Issue 6 Pages 225-231
Published: 1954
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSNasal mucosa is always exposed to the danger of the invasion of microorganisms as the opening of respiratory system, therefore, it is very much likely that this part of the body might possess some particular function which would prevent the invasion of pathogenic agents such as tubercle bacilli. As it was not practicable to inoculate virulent tubercle bacilli into human nasal mucosa, tuberculin injection was made into the nasal mucosa and the reactions produced were examined pathohistologically in order to know some aspect of such defensive function.View full abstractDownload PDF (1697K)
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