肺空洞の病態生理に関する研究

とくに空洞肺組織の化学組成 (燐分画) を中心とする考察-その2実験例について

抄録

Following the previous report (Part I of the study), the author made biochemical analysis on phosphorus fractions of lung tissue in experimental tuberculosis of rabbit with special reference to the changes of the amount of phosphorus fractions with the process of cavity formation. Similar analysis was made on experimental Brown-Pearce lung cancer as a control.
Phosphorus fractions were extracted from cavity wall, its surrounding tissue and healthy tissue of opposite side by the modified Schmidt-Thanhauser's method, and the measurement of each fraction was conducted by the Allen's method. The stage of cavity formation was divided into the following 4 stages, precavitation stage, early stage of cavitation, com pletion stage and regression stage. Change in the amount of phosphorus fractions in these 4 stages were analyzed with special reference to the macro scopic findings of cavity. The results were the following.
1) No significant difference was found in the amount of acid-soluble phosphorus between healthy tissue and tuberculous cavity upto the early stage of cavitation, and in the completion stage, the a mount of ASP showed increase in cavity wall. In the regression stage, the amount of ASP increased mark edly in cavity wall and its surrounding tissue.
2) The amount of phospholipids showed decrease in the precavitation stage in cavity wall and its surrounding tissue, and in later stages, it showed continuous decrease in caseous mass, cavity wall and its surrounding tissue. In healthy tissue, it showed no significant changes in these 4 stages.
3) The amount of DNA showed no significant difference among different parts of lung in the pre cavitation stage, showed increase in caseous mass, in the early stage of cavitation, then decreased in the later stages. The amount of RNA in cavity wall and its surrounding tissue decreased in the precavitation stage, showed normal level in the early stage of cavitation, and again decreased in the later stages.
4) ASP in all parts of lung tissue increased in larger and multilocular cavity and cavity with sur rounding lesions, and ASP in cavity wall and its surrounding tissue showed increase in thick-walled cavity and cavity with few caseous mass.
5) The amount of phospholipids was biggest in healthy tissue, next in surrounding tissue, then in cavity wall and least in caseous mass regardless of macroscopic findings of cavity.
6) DNA showed the same level in all parts of lung tissue regardless of macroscopic findings of cavity, and on the contrary, RNA increased in thin walled and multilocular cavity and cavity with few caseous mass and with surrounding lesions, but no significant difference was found in RNA of different parts of lung tissue.
7) Comparing the amount of phosphorus fractions between tuberculosis and cancer, the latter in creased in all parts of lung tissue. The most marked difference between tuberculosis and cancer was found in the amount of ASP.
8) In case of tuberculosis, ASP was less in case ous mass and cavity wall, while in case of Brown Pearce lung cancer, marked increase of ASP was observed in cancer lesion. In all parts of lung tis sue, no significant difference was found in the amount of phospholipids between tuberculosis and cancer.
9) In case of tuberculosis, DNA showed nearly the same level in different parts of lung tissue, while in case of Brown-Pearce lung cancer, DNA showed increase in cancer lesion and its surround ing tissue. RNA showed increase in healthy tis sue in case of tuberculosis, and on the contrary, it increased in cancer lesion in case of Brown-Pearce lung cancer. The results coincide well with the results obtained in case of resected lung specimens.