INHIBITORY EFFECT OF TUBERCULOGLYCOPROTEIN ON LYSOSOMAL ENZYME ACTIVITY

Abstract

Mixed paracrystal tuberculoprotein-glycoprotein (MCTP-GP) isolated from unheated culture filtrate of the strain H₃₇Rv of Mycobacterium tuberculosis was shown to noncompetitively inhibit the in vitro activity of lysosomal enzymes such as acid phosphatase, N-acethyl-β-glucosaminidase, N-acethyl-β-galactosaminidase, β-glucuronidase, β-galactosidase and α-gluco sidase. The inhibitory activity was the highest against acid phosphatase, the lowest against α -glucosidase and moderate against the other four enzymes. The inhibition was optimal at pH 5.0 to 5.5.