実験的肺肉芽腫症における肺局所遅延型アレルギー反応の解析
Ia+肺胞マクロファージとT細胞亜群の動態
1988 年 63 巻 2 号 p. 121-131
詳細
1988 年 63 巻 2 号 p. 121-131
To clarify the delayed type hypersensitivity reaction induced in rat lungs by heat-killed BCG sensitization, the fluctuations of Ia-positive alveolar macrophages and T-cell subsets in bronchoalveolar lavage fluid (BALF), in blood and in the pulmonary tissue were studied by the use of flow cytometry and immunohistochemical methods.
It was found in BALF that neutrophil leukocytes increased at early stage and lymphocytes and plasma cells reached the peak on seven days after the secondary sensitization. Analysis of T-cell subsets in BALF revealed that the ratio of helper T-cells to nonhelper T-cells increased by 2.24 on seven days, whereas nonhelper T-cells gradually exceeded helper T-cells at late stage. These data corresponded quite accurately with the changes of cell populations in the lung tissues in the process of the granuloma formation, but these changes of T-cell subsets were not found in the peripheral blood lymphocytes.
The granuloma formation in the lungs was seen remarkably at early stage. At the same time, it was also noted that Iapositive alveolar macrophages increased by 46.6% in BALF on seven days and Ia antigens on the epithelioid cells in the granulomas were positive.
These results suggest that the local analysis of cell components in BALF yields dynamic details concerning immune responses of pulmonary regions, and that the cellular interaction between Ia-positive alveolar macrophages and helper Tcells might be important in initiating the delayed type hypersensitivity reaction of the lungs.
