Myoobacterium kansasiiおよびMycobacterium xeopi感染症の治療薬として期待されるSulfadimethoxine
Sulfadimethoxine感受性試験によるM. kansasiiとM. marinumの区別およびM. gordonaeとM. scrqfulaeemの区別
1989 年 64 巻 4 号 p. 313-317
詳細
1989 年 64 巻 4 号 p. 313-317
Susceptilibity testing to sulfadimethoxine of various mycobacteria was made using Ogawa egg medium containing various concentrations of the drug.Each medium was inoculated by a 0.02 ml-sample of bacterial suspensions (10mg wet weight per ml) prepared from 10 day-old (M. tuberculosis, 14 day-old) cultures growing on Ogawa egg medium after homogenizing the bacteria by shaking with glass beads. The media inoculated were incubated at 37°C for 14 days (M. marinum, at 28°C). The minimal inhibitory concentration (MIC) was determined as the lowest drug concentration on which the growth of bacteria was completely inhibited. However, residual growth occurred often. This was regarded as negative growth, because control medium containing no drug always exhibited abundant membraneous growth.
Of the mycobacteria tested, M. kansasii (MICs, 0.8-3.2 μg/ml) and M. xenopi (MICs, 0.2-3.2 μg/ml) were most susceptible to this drug. Other mycobacteria showed the MICs higher than 3.2 μg/ml. The drug seemed to be useful in the treatment of infections caused by M. kansasii and M. xenopi. Furthermore, the susceptibility testing to sulfadimethoxine was considered to be useful for differentiation between two photochromogens, M. kansasii and M. marinum and for differentiation between two scotochromogens, M. scrofulaceum and M. gordonae (Fig.3 and 4).
