抗結核剤の耐性問題第2部: 臨床耐性限界その2. SM, INH, KM, EB
1994 年 69 巻 6 号 p. 397-407
詳細
1994 年 69 巻 6 号 p. 397-407
The proportion method demands an exact count of the colonies on the control and drug- containning media to calculate an exact percentage of resistant bacilli among bacillipopulation.To realize this objective, the bacilli suspension must be fresh and homogeneous, susceptible and resistant bacilli must be equally distributed, each bacillus must be dispersedas a single cell when the bacilli suspension is diluted, colony count must be reduced at a ratepararell to the dilution.
The critical concentration of each drug should essentially be determined in a clinicalmanner, however as the multi-drug combination therapy in the rule for the treatment oftuberculosis, it is very difficult to make an exact determination of the resistance to each ofdrugs.
It may be better, as Canetti pointed out, to decide using the pure bacteriologicalmethod and later compare findings with clinical studies, making changes in the future if sorequired.
We choose to follow their criteria for nearly all of the drugs making only a fewexceptions.
1) SM: DH-SM is not available in Japan as Japanese government prohibits itsproduction. Growth on the medium containing SM (only concentration of less than 4μg/ml) was more abundant than on DH-SM media.
