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Online ISSN : 2434-2386
Print ISSN : 1349-0958
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Structural Analysis of Gastric Proton Pump by Cryo-Electron Microscopy
Kazuhiro Abe
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2010 Volume 45 Issue 4 Pages 264-267

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Abstract

Gastric proton pump, H+,K+-ATPase fulfills the remarkable task to generate very acidic environment (~pH 1) in the stomach lumen, which is indispensable for digestion and also act as first barrier against bacterial and viral infection. On the other hand, too much acid secretion induces acid-related diseases such as peptic ulcer. Therefore, the mechanism by which this massive H+ gradient generated is considerable biochemical interest. In order to address this mechanism, we have shown the three-dimensional structure of H+,K+-ATPase as determined by electron crystallography of two-dimensional crystals, using liquid-helium cooled cryo-electron microscopy. Here we introduce a molecular model for this remarkable biological phenomenon based on the EM structure of H+,K+-ATPase.

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© 2010 The Japanese Society of Microscopy
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