2022 Volume 57 Issue 3 Pages 113-117
About 10 years have passed since the resolution revolution in cryo-EM single-particle analysis (SPA) occurred. During this period, state-of-the-art cryo-EM equipment was rapidly introduced mainly in scientifically advanced countries. Atomic resolution in cryo-EM SPA will be achieved in 2020, and the ripple effect will be even greater. Under these situations, the National Institutes of Natural Sciences (NINS) have introduced a 300 kV cryo-EM, which has recorded the world’s highest performance, and has started joint use by industry, academia, and research institutes in Japan and overseas from FY2022. We will also introduce the latest cryo-FIB-SEM as a cryotomography sample preparation device, and start research aimed at structural analysis of proteins in cells. By combining these with the biomolecular dynamics and function analysis system has been operated at our institutes, we will provide unique research support that seamlessly connects the protein structure and dynamic function. This paper introduces the details and the current status.
