抄録
Electron microscope was applied to the studies of the interaction between the Sh. komagome A phage isolated from the feces of healthy chickens and its host cells.
The samples for electron microscopy were prepared as follows:
The phages are mixed with the susceptible organisms growing in nutrient broth. These broth are incubated for a graded series of time-intervals, and then added the formalin, or chilled in ice water. These preparations are centrifuged and sediments are washed with saline plus formalin. The washed sediments are suspended in water plus formalin and these suspensions are used in the preparation of the samples.
It was found in this experiment that the times rearly corresponding to the half of rising curve of the one-step growth curve would be the best time for the purpose of the preparation for the electron microscopic samples.
