PURIFICATION AND SOME PROPERTIES OF AN ALKALINE RIBONUCLEASE FROM PIG LIVER

Abstract

A method for the purification of an alkaline ribonuclease from pig liver is reported. A 480-fold increase in specific activity has been achived. The enzyme has a pH optimum between 7.7 and 8.0,and the molecular weight is approximately 14,000. The immunological properties of pig liver alkaline ribonuclease are different from those of rat liver alkaline ribonuclease.