トゲシバリとヨコワサルオガゼの地衣体における地衣成分の量的な分布(予報)

抄録

By using the high-speed liquid chromatography (HLC-813d), chemical analyses were made of Cladia aggregata and Usnea diffracta. Three different portions (A=apical, M=middle, B=basaD were taken from the thallus, each portion 1 mg (dry weight). Each sample was prepared by extracting with 2 ml absolute methanol for more than 24 hours at about 60℃. Then, 5 μl of each extract was chromatographed on a column (Flow TSK-GEL type ; 4 mm x 300 mm) with water-methanol (20 : 80,v/v) at 0.8 ml/min. Chromatographic peaks of lichen substances were monitored by absorption of 254 nm, and they were compared with those of authentic substances. The strength of the absorption peak and the concentration of authentic substances (diffractaic acid and barbatic acid) were found to show linear relationship. Therefore, the concentration of each substance was calculated from the strength of the peak (see tab. 1 and 2). Two peaks of Cladia aggregata were identified ; peak b is 4-0-demethyl barbatic acid, and peak c is barbatic acid. Two peaks of Usnea diffracta were identified ; peak e is diffractaic acid, and peak f is usnic acid. Owing to the scarcity the material, the other peaks (a, d, g, and h) were not identified. In the Cladia aggregata and Usnea diffracta examined, the concentration of each of the following substances is highest in the apical portion of the thallus and dereased toward the basal portion : barbatic acid, 4-0-demethyl barbatic acid, usnic acid, and diffractaic acid. In Cladia aggregata the concentration of barbatic acid is remarkably high at the apices of the thallus (see Fig. 1,5). In Usnea diffracta, the concentration of usnic acid is remarkably increased at the apices as barbatic acid in Cladia aggregata, the concentration of diffractaic acid is high in all parts of the thallus and it gradually increases toward the apices. In Cladia aggregata, the ratio of barbatic acid in contrast to the other substances is the highest in the apices, and decreases from the apical to the basal portion of the thallus (Fig. 1). In Usnea diffracta, the content ratio of the diffractaic acid to the other substances is increased from the apical to the basal portion of the thallus. The content ratio of usnic acid in contrast to the other substances decreases from the apical to the basal portion of the thallus (Fig. 2).