Abstract
A rearing method for individual diamondback moth (Plutella xylostella) larvae without diet change was developed. Most of the larvae with a 100mg (or heavier) of leaf of cruciferous plants in a sealed plastic tube (25ml) succeeded in cocooning, pupation and emergence. Newly emerged adults could normally copulate and lay viable eggs. However, in the case of plants leaves that yellowed and/or decayed within 7 to 10 days, most of the P. xylostella larvae did not develop into pupae. This simple and inexpensive rearing method could be used in various bioassays for P. xylostella larvae.