EXPERIMENTAL STUDIES ON THE AMOEBIC LIVER ABSCESS

II. INFLUENCES OF ASSOCIATED BACTERIA AND THE DECREASE OF HOST-RESISTANCE UPON THE PRODUCTION OF THE ABSCESS

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1) Entamoeba histolytica growing in culture media were washed with Ringer's solution by centrifugation to get rid of as many associated bacteria as possible and then they were added with pure culture of strepto- or staphylo-coccus. This mixture of amoebae and bacteria was inoculated directly into the liver of rats. Though abscesses were produced in many of the rats inoculated, amoebae were not found in them at all. As it was considered that the vitality of amoebae might be impaired by the washing with Ringer's solution, pure culture of strepto- or staphylococcus was added directly to the culture of amoebae without preliminary treatment with Ringer's solution. Inoculation of this amoebae-bacteria mixture also did not give a higher rate of the production of amoebic liver abscess as compared with control experiments.
2) Injection of antibiotics after the inoculation much reduced the death of the experimental animals and abscesses produced were small in size and amoebae were found in none of them while in the control animals they were found in 30% of abscesses produced.
3) Strains of amoebae associated with a single strain of Escherichia coli or Clostridium perfringens produced abscesses in rather a small percentage of animals inoculated and amoebae were detected in none of the abscesses.
4) To reduce the resistance of host to pathogenic microbes, CCl or inulin was administered before or after the inoculation of amoebae. Definite effects of these substances were, however, not recognized partly due to the premature death of experimental animals.