The Keio Journal of Medicine
Online ISSN : 1880-1293
Print ISSN : 0022-9717
ISSN-L : 0022-9717
EFFECT OF 2-MERCAPTOETHANOL ON CYANOGEN BROMIDE CLEAVAGE OF PROTEINS
TSUNEJIRO SEKITAGUNJI MAMIYAKIYOSHI SEKITA
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1975 年 24 巻 3 号 p. 203-210

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1. On the cyanogen bromide cleavage of urease in 70% formic acid, addition of 2-mercaptoethanol led to the increase of the cleavage.
2. Dithiothreitol and thiodiglycol were not effective for it.
3. The cleavage ratio was estimated by gel filtration, N-terminal determina tion and the yield of cleaved peptides.
4. A similar effect was observed on phosphorylase b as urease.
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