散乱パターンにおける粒径分布および乱方向性の影響

バクテリア散乱パターン解析のための基礎的検討

抄録

The light scattering patterns are analyzed for polydispersed nonspherical particles with internal structures, with a view toward the optical studies of bacterial species. When the particles are size-distributed and randomly oriented, the extrema of the scattering pattern are smoothed out, the valid range of Rayleigh-Debye approximation is extended, and the scattering model of complex scatterers can be simplified considerably. The smoothed scattering pattern provides information about average sizes, common shapes and common structures of the scatterers, but it does not clearly provide the information about their refractive indices. Outer and inner structures of bacteria affect the forward and backward scattering respectively. Experimental results with latex spheres and nonspherical bacteria show close agreement with the theoretical calculations based on the scattering pattern analysis presented here.