This paper reports a measuring method of the flow velocity using a transmission grating. The flow velocity is determined from the velocity measurement of particle images on the transmission grating Placed in front of a photodetector. These images formed by a microscope objective correspond to particles moving in a flowing fluid. By using the present method, the flow velocity distributions in a small flow cell are measured and their results are compared with the data obtained by using the differential laser Doppler method. It is shown that the present method can provide the data which are comparable in sensitivity and accuracy to the data obtained by using the differential laser Doppler method.