In Vitro Effect of Juvenoid on the Incorporation Activity of Labeled Precursors into Nucleic Acids and Protein under the Incubation of the Posterior Silk Gland of the Silkworm, Bombyx mori

Abstract

I investigated the effect of JHA on the incorporation activity of precursors into nucleic acids and protein of the posterior silk glands under in vitro condition. Respective incorporations of ³H-TTP into DNA and ¹⁴C-leucine into protein were ceased when reaction media were lacking in boiled extract of larval haemolymph and amino acids. RNA synthesis expressed as the uptake of ³H-uridine was not remarkably affected by the JHA addition. JHA repressed somewhat the incorporation of ³H-thymidine into DNA and moreover, it appeared as if the enhancement of the uptake of ³H-TTP into DNA caused by the addition of ATP was negated. The incorporation of labeled glycine into protein was accelerated by the JHA addition, but conversely, that of labeled leucine was remarkably suppressed. Such an inhibitory effect of JHA lasted after exclusion of JHA from the reaction medium. A possibility of concerning of JH with priming activity of DNA was discussed.