ウシ骨基質由来の骨細胞増殖因子の精製とその性質

抄録

The mechanism of the bone formation and remodelling has been investigated by many researchers, however, little is known about the role of the local factors in the regulation of the skeletal growth. In this paper, the author attempted to purify and characterize the factors derived from the bovine bone matrix affecting the proliferation and differentiation of the bone cells.
The non-collagenous protein fractions extracted from the bovine bone with 0. 5M EDT A/1M NaCl contained factors to promote the proliferation of the embryonic chick calvarium cells in culture. Most of this activity was recovered in an acid-insoluble, calcium-induced precipitate fraction. This fraction was purified by DE-52 ion exchange chromatography and the activities were eluted in several peaks. Two of the active peaks were further chromatographed by gel filtration with S-200 and were eluted between 12, 500 and 25, 000 in an apparent molecular size. Both of these partially purified fractions stimulated the proliferation of the embryonic chick periosteal and skin cells, but inhibited that of the osteoblast cell line (MC3T3-E1) . The effect of the alkaline phosphatase activity by those factors was found in the MC3T3-E1 cells as well as in the embryonic chick calvarium cells.
These results suggest a possibility that the multiple factors influencing the proliferation of the osteoblasts may exist in the physiological bone matrix.