抄録
By sensitizing human red blood cells with corresponding antisera specific to blood group antigens and subsequent rosette formation with Staphylococcus aureus, we were able to distinguish homozygosity of the antigens from heterozygosity. Three blood group systems : Rh, Kell and Kidd were used in this study. In the Rh blood group system, we were able to demonstrate the dosage effect of the Rh0 (D) antigen by rosette assay in addition to determining that the deletion of the C and/or E alleles increased the rosette forming cell count when red blood cells with different genotypes were sensitized with anti-D. Our assay proved to be simple, specific, highly reproducible, and can be adapted to study virtually any antigenic markers on a cell surface.
