Study of Acid DNase in Cow Snout Epidermis by Micro Slab Electrophoresis Using Acidic and Basic Gel Sheet
1988 年 35 巻 1 号 p. 1-6
詳細
1988 年 35 巻 1 号 p. 1-6
Electrophoretic profiles of acid DNase in cow snout epidermis were studied electrophoretically in relation to the depth of epidermis. Isoelectrophoretic point of acid DNase protein was found to be 4.9 and 8.4. To examine the electrophoretic properties of acidic and basic enzyme proteins, acid DNase was analyzed by micro slab electrophoresis, using 5% (pH 4.3) and 10% (pH 8.8) acrylamide gel sheet, respectively. Acid DNase was detected throughout all three successive layers of epidermis. In the case of acid DNase (basic protein) analysis carried out on the pH 4.3 gel sheet, the additional minor component was found beside major band, in the upper layer of epidermis.
