Studies of New Methods for Urinary Protein Determination : II. Modified Trichloroacetic Acid (TCA)-Ponceau S Method by Addition of Surface Active Agent (Triton X-100)

Abstract

Modified TCA-Ponceau S method for urinary protein determination by addition of surface active agent (Triton X-100) was studied and compared with Pesce-Strande method, the modified method by Sasaki et al. (1979), biuret method and others. This method was independent of temperature and was not appreciably affected by the albumin/γ-globulin ratio. The correlation coefficient between biuret method and this method was 0.998, and showed high sensitivity and satisfactory reproducibility. The procedure is not one-step and not as simple as Kingsbury-Clark method, but the main advantages of this method are its high and approximately same sensitivity for albumin and γ-globulin, and probably its specificity.