1991 Volume 22 Issue 4 Pages 567-574
The author studied the effects of fluorescein sodium and miotic solutions, such as carbachol and/or acetylcholine, on endothelial function of rabbit cornea by monitoring the transendothelial electrical potential difference (TEPD). As controls for fluorescein, a balanced salts and glucose (BSG) solution was used. The TEPD in a BSG solution increased for the first 30 min and then decreased slowly, reaching about 60 % of its initial value after 5 h. W hen a BSG solution containing 5 μg/ml of fluorescein sodium was used, the TEPD time course was similar to that of the control solution. With a fluorescein sodium concentration of 500 μg/ml, the TEPD decreased below control value after 4 h of exposure. In the case of a miotic solution, a balanced salts solution was used for control. A solution containing 55 mM acetylcholine and minimal amounts of salts maintained a transendothelial electrical potential difference 30 % above control levels for up to four hours. Two other solutions including either balanced salts and 0.55 mM carbachol or a mixture of 0.19 mM carbachol and 55 mM acetylcholine plus minimal salts were adequate to maintain the TEPD at control levels. A solution with acetylcholine without any salts decreased the TEPD to 30 % of the control level in 100 minutes.
