An approach to fixation and embedding of the monolayer cultured cells for electron microscopy using fibrin plate

Abstract

A newly-devised fixation and embedding procedure of the monolayer cultured cells for the electron microscope were discussed. The fibrin plate was first formed over the flask wall. On the surface of the plate the cells were cultured. This resulted not only in a very good growth of the cells, but also in a clear exfoliation of the cultured cells from the TD tube wall without using any drug or causing mechanical damages. This method proved to faciliate fixation and embeddig process saving troublesome procedures, and to make sectioning easier as well.