抄録
By using a primer set of pJF1 and pJR1, polymerase chain reaction (PCR) fragments, ca. 1,300 bp and ca. 2,700 bp were amplified from Pyricularia grisea isolates, Kyu9439013 and OS99-G-7a, respectively. The fragments were found to be unique to each isolate, suggesting that these could be used as specific DNA markers for the 2 isolates. Further investigation confirmed the specificity of the fragments: in the 480 isolates examined, some showed a fragment of ca. 800 bp, while others showed none. The specific markers of the 2 isolates were stably detected after 50 times transfer on medium and 7 times infection on rice leaves. Further, virulence of the 2 isolates was not reduced after the rice infection. Thus, since the isolates Kyu9439013 and OS99-G7a can be clearly identified by a specific DNA marker, these can be used as efficient tools to trace the rice blast pathogen in natural fields.
