Abstract
In October 1998, strawberry fruits rot with black and brown spots was observed in forcing culture in Saga prefecture, Japan. Diseased plants also showed spots on flowers and calyxes. and peduncle rot. Pathogenic fungi were isolated from diseased plants, and these were identified as Colletotrichum acutatum and C. gloeosporioides (teleomorph: Glomerella cingulata) based on conidia shape and size, colony growth, sensitivity to benomil and diethofuencarb, and unique detection of the internal transcribed spacer 1 region of ribosomal DNA. As the result, spots of flowers and calyxes, and rot of fruits and peduncles should be added as symptoms of strawberry anthracnose together with those formerly described in forcing culture in Japan.