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Akira MORITA
2006 Volume 52 Pages
1-5
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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A disease similar to southern blight has been occurring on Farfugium japonicum (L.) Kitam. in fields and gardens in Nagasaki Prefecture since 2003. Fungi similar to Sclerotium rolfsii Saccardo, which is the causal fungus of southern blight, were consistently isolated from collar rot lesions of F japonicum. The isolates were identified as S. rolfsii based on mycelial growth, colony color, sclerotia formation, and pathogenicity against F japonicum. It is proposed that this disease should be referred to as the southern blight of F. japonicum.
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Kazutaka SHINOHARA, Tatsuya YUDA, Hiroyo NISHIMOTO, Akiko HAMASHIMA, S ...
2006 Volume 52 Pages
6-10
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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Citrus Huanglongbing (syn. greening disease) caused by 'Candidatus Liberibacter asiaticus' were surveyed on the Amami islands from 2002 to 2006. A total of 212, 587 Rutaceae plants were surveyed at 29, 063 sites on the Amami islands. Samples that showed a greening-like symptom (17, 384 samples) were examined by a polymerase chain reaction (PCR) assay according to the method of Jagoueix et al. (1994). Of these, 1, 041 (0.5%) samples were identified as being infected with 'Candidatus L. asiaticus.' These infected samples were found on the Kikai-jima, Tokuno-shima, Okierabu-jima, and Yoron-tou islands; however, infected samples were not found in Amami-O-shima (Amami main island).
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Jun-Ichirou YAMAGUCHI, [in Japanese]
2006 Volume 52 Pages
11-17
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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In October 1998, strawberry fruits rot with black and brown spots was observed in forcing culture in Saga prefecture, Japan. Diseased plants also showed spots on flowers and calyxes. and peduncle rot. Pathogenic fungi were isolated from diseased plants, and these were identified as Colletotrichum acutatum and C. gloeosporioides (teleomorph: Glomerella cingulata) based on conidia shape and size, colony growth, sensitivity to benomil and diethofuencarb, and unique detection of the internal transcribed spacer 1 region of ribosomal DNA. As the result, spots of flowers and calyxes, and rot of fruits and peduncles should be added as symptoms of strawberry anthracnose together with those formerly described in forcing culture in Japan.
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Mitsuru Sayama, Tetsuji Ogawa, Yoshihiro Mukaida
2006 Volume 52 Pages
18-23
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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Potato virus Y (PVY) causes serious yield loss to seed potato production in the world. We investigated the seasonal occurrence of four aphid vectors and the infection period of PVY in potato fields in the Shimabara peninsula. Aphis gossypii, Myzus persicae, and Aulacorhtum solani first appeared in the fourth week of March in 2003 and 2004 and in the first week of April in 2005. They were collected when the mean weekly temperature was above 12°C. Macrosiphum euphorbiae was not collected during the survey periods. Virus infection started with the emergence of these vectors in 2005, although it started two or three weeks later in 2003 and 2004. The PVY-infection ratio increased between the fourth week of April and the first week of May. These results suggested that harvesting tubers by the fourth week of April could reduce the PVY infection.
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Yatsuka Nishi, Naoshi Omatu
2006 Volume 52 Pages
24-27
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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Mitsuru Okuda, Sadaharu Tanaka, Kazuya Ikeda, Toru Iwanami
2006 Volume 52 Pages
28-32
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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Cucumber (Cucumis sativus) lines showing resistance to Zucchini yellow mosaic virus (ZYMV) were selected. Two lines (temporarily designated SK-003 and SK-004) showed high resistance to ZYMV, but susceptibility to Papaya ringspot virus (PRSV). SK-003 and SK-004 exhibited a veinal chlorosis on the first and second true leaves when the cotyledon was inoculated with ZYMV. ZYMV was not detected on the fourth leaves, although it was detected from those below the third leaves. A field test confirmed that SK-003 and SK-004 were resistant to ZYMV under natural conditions.
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Takashi Nakajima, Kenta Tomimura, Megumi Yoshida
2006 Volume 52 Pages
33-37
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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Masaya Matsumura
2006 Volume 52 Pages
38-40
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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Suguru Ohno, Tomonori Sasaki, Tsuguo Kohama
2006 Volume 52 Pages
41-43
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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Egg incubation of Euscepes postfasciatus is a necessary step in the mass rearing of this species because its egg period is slightly long (mean, 8 days at 25°C). E. postfasciatus eggs tend to stick to each other during the incubation period. To ensure sufficient disinfection of the egg surface and an even distribution of the eggs on the artificial diet, it is necessary to separate the stuck eggs. The separation process is time-consuming; it can be shortened by ensuring, as far as possible, that the eggs are not in contact with each. The effect of the incubation condition on egg hatching of E. postfasciatus has not yet been examined; thus, we compared the parameters for hatching between eggs incubated en masse (the present method) and those that had been thinly dispersed (a newly tested method). The eggs could be dispersed easily by putting eggs on a well-strained nylon cloth and gently swaying the cloth in water. The hatchability, mean egg period, and variance of the egg period did not vary significantly between the two incubation conditions. The separation time of E. postfasciatus eggs can thus be shortened without any undesirable effects on egg hatching of the species.
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Shigenori Ueda
2006 Volume 52 Pages
44-48
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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The Q biotype of Bemisia tabaci (Genn.) was first identified in Japan in 2004 by nucleotide sequence analyses of the mitochondrial cytochrome oxidase I (mtCOI) gene (Ueda and Brown, 2006). The morphological characters of B. tabaci are not definitive for differentiating biological variants or biotypes. In this report, a simple, rapid method for the identification of the Q biotype without mtCOI gene sequencing analysis is presented. The mtCOI PCR products (866 bp) from the Q biotype were digested with EcoT141 (StyI) into two fragments of 555 by and 311 bp, while the PCR products from the B biotype were cleaved with Stul into two fragments of 560 by and 306 bp. The PCR-RFLP method described here is sufficient for distinguishing populations of the Q biotype in a number of samples obtained from fields.
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Hiroyo Nishimoto, Kazuki Kakimoto, Hideaki Inoue, Tomotoshi Kashio
2006 Volume 52 Pages
49-53
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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Tetsuya Yoshioka, Yukio Andou, Norihito Ogura
2006 Volume 52 Pages
54-59
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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Takuhiro Yamaguchi, Junji Iwamoto, Hideaki Goto, Hidenobu Nojima, Naos ...
2006 Volume 52 Pages
60-65
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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We investigated the insect pests that occurred on mango trees, Mangifera indica, grown in green houses on the Amami islands, Kagoshima, Japan. A total of 6 species of Acarina, 4 species of Thysanoptera, 9 species of Lepidoptera, 11 species of Hemiptera (except 13 species of Coccoidea that had already been reported), and 3 species of Coleoptera that attack the mango plant were found during 1996-2001. Six species-Oligonychus coffeae (Acarina: Tetranychidae), O. biharensis (Acarina: Tetranychidae), Cisaberoptus kenyae (Acarina; Eriophyidae), Selenothrips rubrocinctus (Thysanoptera; Thripidae), Microceropsylla nigra (Hemiptera; Carsidaridae), and Euwallacea fornicatus (Coleoptera; Scolytidae)-that also attack this plant on the Amami islands have been reported for the first time.
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Tsuyoshi Ooishi, Tetsuya Toyosato, Chie Moromizato, Hidekazu Tamashiro ...
2006 Volume 52 Pages
66-70
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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Shuji Hayashikawa, Hiroshi Suenaga, Hiroaki Torigoe
2006 Volume 52 Pages
71-74
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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Keiji Yasuda, Tuyoshi Ooishi, Futoshi Kawamura
2006 Volume 52 Pages
75-78
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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2006 Volume 52 Pages
79-90
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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2006 Volume 52 Pages
90-116
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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2006 Volume 52 Pages
117-120
Published: November 10, 2006
Released on J-STAGE: May 22, 2009
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