マイコトキシンの化学分析に関する研究
Sterigmatocystin及び5-methoxysteringmatocystinのボルタンメトリー検出器を利用する高速液体クロマトグラフィーについて
1986 年 1 巻 2 号 p. 90-95
詳細
1986 年 1 巻 2 号 p. 90-95
Micro determination of sterigmatocystin(Stg)and 5-methoxysterigmatocystin(5-methoxystg)in animal tissue and organs by a method using high-performance liquid chromatography(HPLC)with electrochemical detector was described. Electrochemical detectors consisted of one and twc working electrodes were compared. In the former an optimum potential of the working electrode was 1.34V for Stg and 1.16-1.34V for 5-methoxystg, and in the latter when a potential of first working electrode was set at O.9V an optimum potential of the second working electrode was 1.0-1.34V. As a preferable potential for voltammetric detection should be set at less than 1.OV, it is demonstrated that an electrochemical detector consisted of two working electrodes is better than that of one working electrode. The detection system is useful to analyze Stg and 5-methoxy stg in animal tisses.
