Abstract
Background: Sysmex UF-5000 urine particle analyzer can simultaneously measure urinary organic constituents
and provide bacterial information (BACT-info). Reported here are findings of a retrospective review of BACT-info
bacterial flag information provided by Sysmex UF-5000 and urine culture findings, with focus on bacterial
count.
Patients and methods: A total of 1011 patients were enrolled, including 465 males and 546 females. Sysmex UF-
5000 shows bacterial count as BACT-info, and at the same time estimates bacterial count and classifies the findings
into Gram-positive, Gram-negative, Gram-positive/negative, and Unclassified.
Results: The positive predictive value (PPV) of a BACT-info bacterial count ranging from 100-99,999/μL and
urine cultures was 40.1% for all Gram-positive samples, and after dividing UF-5000 positive samples into groups
A (100-999/ μ L), B (1000-9999/ μ L), and C (10,000-99,999/ μ L) was 34.4%, 46.2%, and 48.4%, respectively
(P < 0.05). For Gram-negative samples, the PPV was 86.0% overall, and 52.1%, 87.8%, and 95.5% (P < 0.001) for
Groups A, B, and C, respectively. In both Gram-positive and -negative samples, the PPV increased significantly
with higher bacterial count.
Conclusions: A higher BACT-info bacterial count leads to a more accurate determination of bacteria, suggesting
that BACT-info could lead to appropriate selection of antimicrobial agents in the treatment of urinary tract infections.
