フーリエ変換非線形分光法を用いた蛍光タンパク質の 光褪色スペクトルの計測

須田 亮; 高橋 弘史; 戸田 圭亮

doi:10.2184/lsj.43.4_213

抄録

We present measurements of photobleaching spectra of fluorescent proteins using nonlinear Fouriertransform spectroscopy with ultrabroadband femtosecond pulses. The photobleaching of enhanced green fluorescent protein occurs through sequential three-photon process; one-photon excited-state absorption following two-photon excitation from the ground state. On the other hand, the photobleaching of redform Kaede shows complicated features occurring through sequential three-photon process or sequential four-photon process. The latter corresponds to two-photon excited-state absorption following twophoton excitation from the ground state.

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