Abstract
Delivery of exogenous molecules into live cells is one of the most essential techniques in current
molecular biology and biomedical applications. Among various techniques, optical injection by the use
of ultrashort pulse laser has proven to be an effective approach for targeted molecular delivery and
transfection into single cells. Focused ultrashort laser pulse onto a cell membrane through a microscope
objective creates a sub-micron temporal pore, and exogenous molecules enter to a cytosol. Recent
decades have seen the development of the delivery of exogenous molecules into a single mammalian cell
(NIH3T3) and plant cell (Tobacco BY-2) using a femtosecond Ti:Sapphire amplifier. In this review, we
demonstrate the delivery of various molecular tools such as DNA plasmids and mRNA for genetic
engineering into a targeted single cell in the embryos of various animals is possible.
