The Review of Laser Engineering
Online ISSN : 1349-6603
Print ISSN : 0387-0200
ISSN-L : 0387-0200
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Volume 45, Issue 11
Special Issue on Roles of Optics and Laser Technology in Molecular Delivery
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Special Issue on Roles of Optics and Laser Technology in Molecular Delivery
Special Issue
Laser Review
  • Preface to Special Issue on Roles of Optics and Laser Technology in Molecular Delivery
    Shunichi SATO
    2017 Volume 45 Issue 11 Pages 684-
    Published: 2017
    Released on J-STAGE: December 17, 2020
    DOIhttps://doi.org/10.2184/lsj.45.11_684
    JOURNAL FREE ACCESS
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  • Molecular Delivery to a Targeted Cell with Near-Infrared Ultrashort Pulse Laser
    Ryohei YASUKUNI, Yoichiroh HOSOKAWA
    2017 Volume 45 Issue 11 Pages 686-
    Published: 2017
    Released on J-STAGE: December 17, 2020
    DOIhttps://doi.org/10.2184/lsj.45.11_686
    JOURNAL FREE ACCESS
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    Delivery of exogenous molecules into live cells is one of the most essential techniques in current molecular biology and biomedical applications. Among various techniques, optical injection by the use of ultrashort pulse laser has proven to be an effective approach for targeted molecular delivery and transfection into single cells. Focused ultrashort laser pulse onto a cell membrane through a microscope objective creates a sub-micron temporal pore, and exogenous molecules enter to a cytosol. Recent decades have seen the development of the delivery of exogenous molecules into a single mammalian cell (NIH3T3) and plant cell (Tobacco BY-2) using a femtosecond Ti:Sapphire amplifier. In this review, we demonstrate the delivery of various molecular tools such as DNA plasmids and mRNA for genetic engineering into a targeted single cell in the embryos of various animals is possible.
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  • Photo Responsive Drug Delivery System (DDS) Using Mesoporous Silica
    Masahiro FUJIWARA
    2017 Volume 45 Issue 11 Pages 691-
    Published: 2017
    Released on J-STAGE: December 17, 2020
    DOIhttps://doi.org/10.2184/lsj.45.11_691
    JOURNAL FREE ACCESS
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    Targeted drug delivery system (DDS) triggered by photo irradiation is described in this paper. A mesoporous silica with a coumarin substituent grafted to the outlet region can store a drug molecule in its mesopore, when the coumarin is dimerized by the irradiation of longer wavelength UV light to close the gate of the mesopore. The drug molecule carried to target part can be released from the mesopore by the cleavage of the coumarin dimer triggered by the irradiation of shorter wavelength UV light. The continuous photo isomerization of azobenzene induced by the simultaneous irradiation of UV and visible light promotes the release of a drug molecule from the mesopore. Double modified mesoporous silica with the coumarin and the azobenzene substituents realizes an advanced targeted drug delivery. Using this silica, both the release and the release rate of drug molecule in the mesopore can be controlled by photo irradiations.
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  • Photoresponsive Gene Delivery System: Photoregulation of Intracellular Trafficking Using Photoresponsive Liposome
    Takeshi NAGASAKI, Tsutomu HAMADA
    2017 Volume 45 Issue 11 Pages 696-
    Published: 2017
    Released on J-STAGE: December 17, 2020
    DOIhttps://doi.org/10.2184/lsj.45.11_696
    JOURNAL FREE ACCESS
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    Since light as a stimulus can be controlled both temporally and spatially, photo-irradiation improves both the endosomal escape of exogenous DNA and DNA release from the complexes with carriers. When a photo-responsive cationic lipid formed a cell-sized giant vesicle, we observed the photo-isomerization effect of azobenzene on its shape change. Photoswitching the membrane line tension induced reversible transitions between a closed sphere and an opened disk with a bilayer pore. In a transfection experiment using a photoresponsive cationic lipid, UV irradiation improved the transfection efficiency. After the lipoplexes passed through the plasma membrane by endocytosis, trans to cis isomerization of azobenzene moiety destabilized the vesicle membrane, thus accelerating not only the membrane fusion of the photoresponsive lipid vesicles but also the endosomal escape of the exogenous DNA.
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  • Optical Control of the Structure and Function of Lipid Bilayers by Plasmonic Nanomaterials
    Tatsuya MURAKAMI, Hirotaka NAKATSUJI
    2017 Volume 45 Issue 11 Pages 701-
    Published: 2017
    Released on J-STAGE: December 17, 2020
    DOIhttps://doi.org/10.2184/lsj.45.11_701
    JOURNAL FREE ACCESS
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    Cell function and behavior can be controlled by light. With“ optical switches”, such as dye molecules, rhodopsins, and plasmonic nanomaterials, more precise and less invasive control of them would become possible. Here, I briefly summarize recent advance in the development of optical switches and explain how they manipulate cell function and behavior upon illumination while introducing our optical switch developed on the basis of intracellular drug delivery system.
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  • Antibacterial Activities of Silver Nanoplates Controlled by Pulsed Laser
    Hiroaki ICHIMARU, Kaung KYAW, Takayuki KAWAGOE, Mitsuhiro TERAKAWA ...
    2017 Volume 45 Issue 11 Pages 705-
    Published: 2017
    Released on J-STAGE: December 17, 2020
    DOIhttps://doi.org/10.2184/lsj.45.11_705
    JOURNAL FREE ACCESS
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    Silver nanoplates coated with gold, when subjected to pulsed laser irradiation, changed their shape from triangular to spherical structure along with the shift of their extinction spectra. Simple crystal structure of the silver nanoplates changed to multiple small crystal domains. The ratio of silver to gold on the surface also changed from 22:1 to 4.5:1 enabling more silver to be released. As a result, the antibacterial activity of gold-coated silver nanoplates was significantly increased after pulsed laser irradiation.
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  • Theranostics for Drug Delivery Based on Short Pulsed Light-Induced Photoacoustic and Photomechanical Effects
    Yasuyuki TSUNOI, Satoko KAWAUCHI, Mitsuhiro TERAKAWA, Shunichi SAT ...
    2017 Volume 45 Issue 11 Pages 709-
    Published: 2017
    Released on J-STAGE: December 17, 2020
    DOIhttps://doi.org/10.2184/lsj.45.11_709
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    We have developed a theranostic system that enables both drug delivery and imaging of drug distribution for efficient and side effects-free pharmacological therapy. In this system, nanosecond light pulses are used both for photoacoustic (PA) imaging and drug delivery. With low-energy light pulses, thermoelastic waves (PA waves) are induced in tissue for imaging blood vessels as well as drug distribution, while strong pressure waves called photomechanical waves (PMWs) are generated by irradiating a lightabsorbing material with high-energy light pulses to increase the permeability of blood vessels for targeted transvascular drug delivery. Conditions for drug delivery can be adjusted on the basis of information on blood vessel structures and drug distribution with the system. In this article, we describe the principle, design and characteristics of the system. The system was applied to drug delivery to tumors in mice. The results are also reported.
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Regular Paper
Laser Original
  • Instantaneous Frequency Control within a Single Pulse Period Using Pulsed Serrodyne Modulation
    Eisuke HARAGUCHI, Hitomi ONO,, Toshiyuki ANDO
    2017 Volume 45 Issue 11 Pages 714-
    Published: 2017
    Released on J-STAGE: December 17, 2020
    DOIhttps://doi.org/10.2184/lsj.45.11_714
    JOURNAL FREE ACCESS
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    We developed a new pulsed-coherent light source for wind sensing by combining a semiconductor optical amplifier (SOA) with a phase modulator that was operated to produce pulsed serrodyne modulation. Even though an SOA-based light source has the potential advantages of a small footprint, monolithic integration, and gain properties, instantaneous frequency deviation inevitably occurs, leading to measurement errors of wind velocity. We demonstrated the correction of such frequency deviation using phase modulation with a pulsed sawtooth waveform and clearly demonstrated that the instantaneous frequency deviation was compensated suffi ciently to achieve residual frequency errors of 0.4 MHz, compared to 40 MHz without correction.
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