細胞レベルでのマルチモーダルディジタルホログラィックイメージング
2019 年 47 巻 5 号 p. 253-
詳細
2019 年 47 巻 5 号 p. 253-
This review paper introduces a new type of microscope that enables simultaneous recordings of phase and fluorescence data and retrieves them in three-dimensions. Conventional microscopes take fluorescence and phase information in sequential manner, and it is far satisfying for fast reactions in living cells. In both imaging, digital holographic techniques are applied. For phase imaging, typical Mach-Zehnder interferometer is applied whilst common-path off-axis fluorescence digital holography is applied to fluorescence imaging. Individual and combined methods were verified by experiments results. We believe that the concept of multimodal digital holographic microscopy and the proposed method will push forward the life science to unprecedented level.
