抄録
Second-harmonic-generation (SHG) microscopy is an attractive imaging modality for the in situ visualizations
of collagen fiber in tissues. For example, collagen fibers in dermis can be visualized as the optical
sectioning of 3D images with high selectivity, good image contrast, high spatial resolution, and moderate
penetration without staining. Furthermore, the image analysis of SHG images enables us to obtain
the quantitative parameters of collagen synthesis, assembly, and distribution; polarization analysis gives
additional information in terms of microscopic fiber orientation. In this paper, we introduce our recent
research progress and discuss the advantages of SHG microscopy in skin measurements.
