超解像ライブイメージング顕微鏡SCLIM の開発とSCLIM の4D 多色 ライブセルイメージングによるゴルジ体タンパク質輸送機構の解明

抄録

In order to directly visualize the events occurring in living cells, a cutting-edge technology has been long awaited that enables simultaneous multi-color and four-dimensional observation with high spatial and temporal resolution. Super-resolution microscopy methods so far available, for example, stimulated emission depletion microcopy (STED), photoactivated localization microscopy/stochastic optical reconstruction microscopy (PALM/STORM), and Structured illumination microscopy (SIM), provide great spatial resolution, but are not sufficient in temporal resolution for live cell imaging. We have developed a unique technology, the super-resolution confocal live imaging microscopy (SCLIM), which achieves the performance required. In the field of cell biology, a big debate arose between two models for explaining cargo transport across the Golgi apparatus: the vesicular transport model and the cisternal maturation model. By using SCLIM, we have conducted simultaneous 3-color high-spatiotemporal visualization of secretory cargo together with early and late Golgi resident proteins. Secretory cargo is indeed delivered within the Golgi by cisternal maturation.