屈折作用による光量変調を適用した 新規輪帯照明法によるiPS 細胞観察

抄録

We proposed a microscopy method for observing phase objects without halos or directional shadows. The key optical element is an annular aperture at the front focal plane of a condenser with a larger diameter than one used in standard phase contrast microscopy. The light flux passing through the annular aperture is changed by the specimen’s surface profile, and then it passes through an objective and contributes to image formation. Images of colonies, formed by induced pluripotent stem (iPS) cells using this method, are compared with the conventional phase contrast method. Our method obtains phase images of iPS cell colonies with clear outlines and their three-dimensional nature