Abstract
Imaging flow cytometry (iFCM) combines the spatial resolution of optical microscopes with the
fast-sampling capability of flow cytometry. In contrast to recent advances in 2D-iFCMs for both analysis
and cell sorting, the throughput and flow-speed in 3D-iFCM were limited. In this review, we overview
the field, explain the challenges in speeding up 3D-iFCM, and report the recent developments of
two 3D-iFCMs based on an idea of acquiring multiple optical sections of cells in a single frame of a pixel-
arrayed camera. The two 3D-iFCM techniques demonstrate a record throughput in cells/sec and a record
speed in meter/sec, respectively.
